| 质粒名称: | pTRE Tight ArchT-GFP |
|---|---|
| 出品公司: | addgene |
| 目录编号: | 79627 |
| 存储实验室: | Yasunori Hayashi |
|
载体骨架基本信息 |
|
| 载体名称: | pTRE-Tight |
| 载体出品公司: | Clontech |
| 空载体大小: | 2605 bp |
| 完整质粒大小: | 2605 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | ArchT |
| 物种来源: | 光合细菌 Rhodopseudomonas palustris |
| 插入大小: | 1668 bp |
| 基因突变: | ArchT is a more sensitive version of Arch (archaerhodpsin; light-driven outward proton pump) |
| GenBank编号 | HM367072.1 |
| 启动子: | Tet responsive Ptight promoter |
| 标签/融合蛋白: | EGFP (C terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI(未破坏) |
| 3’克隆位点: | ClaI(未破坏) |
| 5’测序引物 | AGGCGTATCACGAGGCCCTTTCGT |
| 3’测序引物 | tattaccgcctttgagtgagctga |
|
文献引用方法 |
|
| 材料和方法部分: | pTRE Tight ArchT-GFP was a gift from Yasunori Hayashi (Addgene plasmid # 79627 ; http://n2t.net/addgene:79627 ; RRID:Addgene_79627) |
| 参考文献部分: | Inhibiting the Activity of CA1 Hippocampal Neurons Prevents the Recall of Contextual Fear Memory in Inducible ArchT Transgenic Mice. Sakaguchi M, Kim K, Yu LM, Hashikawa Y, Sekine Y, Okumura Y, Kawano M, Hayashi M, Kumar D, Boyden ES, McHugh TJ, Hayashi Y. PLoS One. 2015 Jun 15;10(6):e0130163. doi: 10.1371/journal.pone.0130163. eCollection 2015. PONE-D-15-13315 [pii] PubMed 26075894 |
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